Journal: Myeloid cells

Article Title: Scalable generation of pure CD103 + cDC1 from iDC1 cultures

doi: 10.70401/mc.2026.0012

Figure Lengend Snippet: WT or Irf8+32 −/− mice received CTV-labeled OT-I and OT-II T cells, and a subset of Irf8+32 −/− mice additionally received iDC1 intravenously. One day later, mice received OVA-pulsed β2m −/− splenocytes or vehicle alone intravenously and were analyzed four days later. (A) Experimental scheme. (B, C) Representative contour plots showing (B) CD45.1 and Vα2 expression among CD8 + T cells, with gates identifying OT-I cells, and (C) CellTrace Violet (CTV) and CD44 expression among OT-I cells, with gates identifying proliferated activated OT-I cells. (D, E) Quantification of (D) OT-I cells as a percentage of live cells and (E) total proliferated activated OT-I cells per spleen. (F–I) Corresponding analysis of OT-II cells. Representative contour plots show (F) CD45.1 and Vα2 expression among CD4 + T cells, with gates identifying OT-II cells, and (G) CTV and CD44 expression among OT-II cells, with gates identifying proliferated activated OT-II cells. (H, I) Quantification of (H) OT-II cells as a percentage of live cells and (I) total proliferated activated OT-II cells per spleen. (D, E, H, I) Data are combined from two independent experiments, each including 1–3 mice per experimental condition, with four mice total per antigen-treated condition and 2–3 mice total for no-antigen controls. * p<0.05, ** p<0.01; NS, not statistically significant (two-tailed unpaired Student’s t-test).

Article Snippet: OT-I T cells were purified as described above, whereas OT-II T cells were purified with the Mouse CD4 + T cell Isolation Kit (Miltenyi Biotec #130-104-454).

Techniques: In Vivo, Labeling, Expressing, Analysis, Two Tailed Test, Flow Cytometry